Primer

Part:BBa_K4047013

Designed by: An Nguyen   Group: iGEM21_MiamiU_OH   (2021-09-08)


dSBPase_glpX_DS-fwd

Forward glpX_DS for SBPase

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Long Description

This is the forward primer to amplify the downstream (3’) portion of the gene glpX encoding sedoheptulose- 1,7-bisphosphatase (Synpcc7942_0505). The overlap sequence (red) allows proper Gibson assembly with the kanR sequence within pGEM-SBPase. The annealing portion (all caps) allows annealing to the WT S. elongatus PCC 7942 genome for proper amplification. The amplicon would be used for the composite part pGEM-SBPase.

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Categories
Parameters
None